Hydrophilic Interaction Liquid Chromatography to Characterize Nutraceuticals and Food Supplements Based on Flavanols and Related Compounds

Purified extracts from different types of berries and medicinal plants are increasingly used as raw materials for the production nutraceuticals dietary supplements, mainly due to their high content in bioactive substances. This is, instance, case phenolic compounds such flavonoids, which exhibit a wide range beneficial properties, including antioxidant, anti-inflammatory, antineoplastic antimicrobial activities. paper tackles characterization several kinds based on hydrophilic interaction liquid chromatography (HILIC) with fluorescence detection (FLD). The study focused determination flavanols related condensed tannins. Analytes were recovered by solvent extraction using methanol:water:hydrochloric acid (70:29:1 v:v:v) under sonication 30 min at 55 °C. Experimental design response surface methodology was optimize HILIC separation achieve good resolution main components, acetonitrile:acetic (99:1 v/v) (solvent A) methanol:water:acetic acid, (95:3:2 v/v/v) B) components mobile phase. For assessment elution gradient, factors B percentage gradient time. best conditions achieved 10% initial linear reach 25% B. Principal component analysis partial least square-discriminant characterize compare compositional features supplements both targeted non-targeted approaches. Results revealed that sample distribution relied oligomeric nature descriptors.